Synthesis of Titanium Oxide Nanoparticles by Sol-Gel Method and Investigation of Physicochemical and Antibacterial Properties of Them on Prokaryotic and Eukaryotic Cells

The objective of this research was to develop a novel method for the synthesis of colloidal solutions of titanium dioxide nanoparticles with high stability and life span.

Based on mentioned points, the issue of this study is the synthesis of nanoparticles via chemical reduction process. The morphologies, compositions, and physicochemical properties of the prepared samples were characterized by TEM, XRD and DLS. Also, the cytotoxic effect of fabricated NPs against human white blood cells (WBCs) was investigated via MTT assay. In addition, antibacterial activity was investigated.

The results of this study indicate that the diameter of the synthesized nanoparticles is about 50nm and contains the anatase phase, in the range of 2θ from 25-80°C, and the hydrodynamic radius of nanoparticles is about 95.8±12.78nm and the zeta potential of nanoparticles is about -34.87±4.78mV. Also, the effect of toxicity of titanium dioxide nanoparticles on the white blood cell line showed that these nanoparticles cause the toxicity of cells at concentrations above 200μg/ml, but in lower concentrations, normal cells can survive. Also, these nanoparticles at the same low concentrations.

In conclusion, colloidal solutions with high stability were successfully synthesized, which, in addition to increasing the antibacterial properties due to diminished dimensions.