Fast protein liquid chromatography

Fast protein liquid chromatography (FPLC) is a form of medium pressure chromatography originally developed for purifying proteins with high resolution and reproducibility. Its distinguishing feature is that the stationary phase is composed of small-diameter beads (generally cross-linked agarose) that are packed in glass or plastic columns and have high loading capacity. The FPLC system allows the use of a wide range of aqueous buffers (the mobile phase) and different stationary phases to perform the main chromatography modes (ion exchange, gel filtration, affinity and hydrophobic interaction, reverse phase). However, anion exchange and gel filtration chromatography are the modes most commonly used.