Introduction to calibrating Transmission Electron Microscope (TEM)

This article provides an introduction to calibrating transmission electron microscopes (TEM). If at any time microscopists suspect that something has gone wrong TEM results, a few quick measurements with a calibration sample will immediately verify or nullify the suspicions. There are three major calibrations for TEM’s. Magnification calibration (images), Camera constant calibration (diffraction patterns), Image/ diffraction pattern rotation calibration (relationship between the images and diffraction patterns). Instructions for each are represented.