In vitro plant regeneration through direct organogenesis in Cordia myxa L.

In this study, a rapid and efficient protocol was developed for in vitro plantlet regeneration of Lasora (Cordia myxa L.) using different explants. Seperate experiments, one for shoot regeneration optimization, one for proliferation, and one for induction were conducted in this study. Factorial design 3*7 based on compeletly randomized design was used for shoot regeneration but just compeletly randomized design used for proliferation and induction. The highest frequency of shoot regeneration (4.33) from cotyledon was obtained on MS medium supplemented with 4.0 mg/l kinetin (KIN) and 0.01 mg/l 1-Naphthaleneacetic acid (NAA). The explants started regenerating shoots after 15-21 days in culture. To proliferation the regenerated shoot, the explants were transferred to MS medium containing different concentrations of KIN or BAP with 0.01 mg/l NAA. Shoot multiplication and elongation took place on the same medium. Indole-3-butyric acid (IBA) at 1.0 mg/l was most effective for root regeneration. Using the current protocol, it tooks 2 months to regenerate plantlets. The in vitro regenerated plantlets were successfully acclimatized and established in greenhouse conditions.