Detection of polymorphism in IGF-I gene and its association with condition factor in the Persian sturgeon fish (Acipencer persicus)

The goal of this study was to detect polymorphisms in Insulin like growth factor-I gene of Persian sturgeon fish (Acipencer persicus) using PCR-SSCP technique and also to investigate their association with fish growth traits (condition factor, body length & weight). In this research, 95 Persian sturgeons were randomly selected and fin clip for DNA extraction were taken from caudal fin. DNA was extracted using modified salting out method and two fragments of 171 and 362 bp in 5/-UTR and 3/-UTR regions of IGF-I gene were amplified, respectively. Genotyping of individuals showed three different banding patterns of M, K and G for the fragment of 171 and 362 bp with the frequency of 13, 39, 48 and 31, 49, 20 percent in Persian sturgeon samples, respectively. The genotype K at 3/-UTR and genotype M at 5/-UTR marker site were the most frequent genotypes. Marker-trait analysis using SAS statistical software indicated no significant association between observed banding patterns of marker loci and growth traits in Persian sturgeon. Considering the important role of IGF-I as a probable candidate gene effective on growth related traits, survey of this marker sites are recommended for larger-size populations