Effects of salinity stress and seed inoculation with bio fertilizers on germination parameters, K and Na content of rye (Secale cereal L.) seedling

In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse respectively at faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabil in 2016. Experimental factors were included soil salinity in four levels (no-salt as control, salinity 25, 50 and 75 mM as NaCl) and seed inoculation with plant growth promoting rhizobacteria in four levels (no inoculation as control, inoculation with Pseudomonas, Azosprilium, both application of Pseudomonas and Azosprilium). The results showed that maximum germination components (such as ridicule and plumule length, radicule and plumule dry weight, germination percentage, uniformity of germination) were obtained at co-application of Pseudomonas and Azosprilium under non-saline condition and minimum of these parameters were obtained at 75 Mm NaCl and no inoculation. K/Na ratio in root and shoot were decreased with increasing salinity level. It was vise versa in seed inoculation with plant growth promoting rhizobacteria. Maximum ratio of Na/K was obtained at the highest level salinity and no inoculation seed with PGPR and minimum of it was obtained in both inoculation with Pseudomonas and Azosprilium and the least level of salinity. It seems that application of PGPR can be used as a proper method for increasing K /Na ratio, germination components and seedling growth of rye under salinity stress.