Study of the effect of some nanoparticles on control of the agent of Calonectria pseudonaviculata under In Vitro conditions

The purpose of this study was evaluation the effects of nanoparticles (nano-cellulose, nano-chitin, nano-chitosan, nano-silver and nano-copper) on the control of disease fungal agent in-vitro conditions.Factorial experiment was carried out in a completely randomized design with three replicates and five concentrations (0, 50, 100, 150 and 200 ppm) for each nanoparticles. Results showed that the using of nanoparticles with the mentioned concentrations in inhibiting the growth of mycelium fungi was significant in box blight and nanoparticles had a significant effect on the growth of mycelial fungi growth. The results of comparison of meanings showed that nano-silver had the most effect and nanocellulose had the least effect on reducing the colonization diameter of fungi. Among the various concentrations tested, the concentration of 200 ppm had the highest effect on the growth of the colony of the fungus, while the concentration of 50 ppm has the least inhibitory growth of of the colony of the fungus.The results showed that application of different concentrations of nano-cellulose and nano-chitin had little effect on inhibiting the growth of colony of fungus caused by Calonectria pseudonaviculata. Meanwhile, nano-copper, nano-silver and nano-chitosan were able to inhibit growth of 200 ppm in 83.1%, 78.8% and 64.9%, respectively. Results showed that using nanoparticles with mentioned concentrations had significant inhibitory effects on fungal mycelial growth. The results of mean comparison showed that nano-silver had the most effect and nano-cellulose had the least effect on reducing fungal colony diameter. 200 ppm concentration had the highest effect with maximum fungal growth inhibition. On the other hand, 50 ppm concentration showed the least inhibitory effect on fungal mycelial growth. The results showed that using of nano-cellulose and nano-chitin different concentrations had little effect on fungal colony growth inhibition.