An evaluation to survey the antagonistic effects of Lactobacillus acidophilus and Lactobacillus plantarum against E. cowanii isolated from plants

Enterobacter are found in water, sewage and plants. Lactobacillus  are one of the dominant microorganisms in normal flora of intestinal tracts, genital system and respiratory tracts of humans and animals. The aim of this study was to evaluate the antagonistic effects of Lactobacillus acidophilus and Lactobacillus plantarum against Enterobacter cowanii isolated from broad bean plant.

In this sectional study Fava plants were collected, and after sampling, the samples were cultured on selective and specific King B  media. The presence of E. cowanii was confirmed based on biochemical and molecular techniques. Then, antagonistic effects of produced bacteriocins by L. plantarum and L. acidophilus on E. cowanii with well diffusion method on basis of pH were studied.

Based on this study, the growth inhibition zones produced by L.plantarum in different pH against E.cowanii were higher than that by L.acidophilus. Furthermore, the highest inhibition zone of Lactobacilli was observed in pH 4. 

According to antagonistic effects of lactic acid bacteria in the inhibition of E.cowanii, this factor can be used for treatment of the diseases caused by this factor.