Morphological and molecular identification of the microalgae ‎affecting on bad smell and unsavory production in Garmsar ‎drinking water

Drinking water of city of Garmsar runs into large pools before being filtered. Recently, due to the ‎growth of algae and their blooms in water surface, following the mortality of many algae, the water ‎became unsavory with bad smell. Deterioration of algae causes the accumulation of a wide variety of ‎organic material which provides a suitable condition for the growth of bacteria, insects, and fungi. To ‎study the algal flora of the pools water samples were collected randomly from different parts and ‎depths of the Garmsar water plan pools, in order to suggest solution for blooming problem. The algae ‎were purified after growing on Cu10 culture media. After two weeks, the species were identified ‎according to‏ ‏valid key of Komárek et al., 2014. Morphologic variation of samples investigated to ‎determine their taxonomic condition in Cu10 culture media. Moreover, morphometric and genetic ‎‎(16S rRNA) data were used to characterize the cyanobacteria strains in liquid suspension cultures and ‎solid media under photoautotrophic conditions. Results demonstrated that the studied strains belong to ‎three divisions of cyanophyta, chlorophyta, and bacillariophyta which includes Anabaena, ‎Synechococcus genera belong to cyanophytae, Ulothrix genus from chlorophyta, and Diatoma from ‎bacillariophyta. ‎Given the presence of microalgae in Garmsar drinking water and‏ ‏their associated risk, the molecular ‎methods are suggested as a reliable and accurate method in addition to morphological analysis in ‎order to identify the types of algae that form into harmful algal bloom.‎