Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on Antioxidant Enzymes Activity of Seedling and Germination Indices of Two Wheat Cultivar Under Salt Stress Conditions

In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely randomized design with three replications in a laboratory of Islamic Azad University branch of Ilam, in 2017. Experimental factors included salt stress at four levels: zero (Control), 50, 100 and 200 mM sodium chloride, PGPR bacteria in two levels: non-inoculation with PGPR (control) and seed inoculation with PGPR and wheat cultivars include: Tajan and Ofogh. The genus and species of PGPR bacteria used in this study included Azotobacter chroococcum, Azospirillum berasilense and Pseudomonas potida, which were obtained from the Iranian soil and water research institute. Experimental traits included Activity of superoxide dismutase enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPX), dry weight of radicl, gomul and seedling, germination percentage, germination rate and seed vigor index. The main effect of experimental factors on all traits was highly significant (P≤0.01). The results showed that Ofogh was superior to Tajan cultivar for all germination indices, dry weight of radicl, gomul and seedling, Activity of antioxidant enzymes. With increasing salt stress, all of traits were decreasing. Priming seeds with PGPR bacteria increased the traits studied. In general, priming of wheat seeds with PGPR in comparison with control treatment (non-inoculation) by improving germination indices and Activity of antioxidant enzymes to some extent reduced the harmful effects of salinity stress.