Effect of Putrescin and Calcium Nitrate on the activity of some antioxidant enzymes and cell membrane stability in sesame (Seasamum indicum L.) under moisture stress

In order to study the effects of application of Putrescin and Calcium Nitrate on some physiological and biochemical traits of sesame under moisture stress a split-plot factorial experiment was conducted based on block complete randomized design with three replications in research farm of university of birjand in 2018. In this experiment, Moisture stress treatments with tree levels were (100, 75, and 50) percent of sesame water requirement as main factor and foliar application Calcium nitrate at tree levels (0, 5, 10 Mm) and Putrescine in two levels (0.5Mm, spray with water) as the second factor were studied. Results showed that interaction of moisture stress, calcium nitrate and putrescine were significant for catalase and polyphenol oxidase activity. The highest catalase activity was obtained in 10 Mm calcium nitrate foliar application at 50% humidity stress and highest activity of polyphenol oxidase enzyme was obtained in 5 Mm calcium nitrate foliar application with 75% of putrescine. Interaction of calcium nitrate and putrescine, moisture stress and putrescine as well as moisture stress and calcium nitrate on peroxidase enzyme and membrane stability were significant. In general, foliar application of calcium nitrate and putrecine was the best treatment to reduce the effects of moisture stress on sesame indicating that there is a synergistic relationship between them in reducing the effects of moisture stress.