Immunogenicity of the Recombinant Cryptococcus neoformans HSP70, a Potential Candidate for Developing an ELISA Kit

Author(s):

Shima Safaei , Hossein Rezvan* , Roohollah Fateh , Mohammad Khalifeh Gholi

Message:
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Cryptococcus neoformans is an encapsulated fungal pathogen that causes life-threatening meningoencephalitis in immunocompromised patients. This yeast secretes several potent immunogenic proteins by secretory vesicular mechanisms, such as HSP70 chaperone.

Methods

The PCR-amplified HSP70 gene was cloned into a PET-28a(+) expression vector. The purified recombinant HSP70  (rHSP70) was evaluated by western blotting using an anti-His Tag-HRP antibody and then used for immunization of a rabbit. The serum of the immunized rabbit was tested against the whole lysate of C. neoformans in ELISA.

Results

The antibodies in the rabbit’s serum recognized lysate of C. neoformans yeast. The highest antibody levels were achieved after the third booster injection.

Conclusion

The rHSP70 showed to be a reliable candidate for the designing and development of an ELISA kit for early detection of cryptococcosis, and to screen a large number of specimens.

Keywords:

Cryptococcus neoformans , Enzyme-Linked Immunosorbent Assay , Heat Shock Protein 70KD

Language:
English
Published:
Journal of Medical Microbiology and Infectious Diseases, Volume:8 Issue: 2, Spring 2020
Pages:
60 to 64
https://www.magiran.com/p2164232  
سامانه نویسندگان
  • Rezvan، Hossein
    Corresponding Author (2)
    Rezvan, Hossein
    (1386) دکتری ایمنی شناسی، دانشگاه بوعلی سینا
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