Phototherapy and Sonotherapy of Melanoma Cancer Cells Using Nanoparticles of Selenium-Polyethylene Glycol-Curcumin as a Dual-Mode Sensitizer

As an alternative form of cancer therapy, photothermal therapy (PTT) and sonodynamic therapy (SDT) using nanomaterials are in development. Nanomaterials can act as energy absorber as well as anti-cancer agent. In this study, the effects of laser and ultrasound irradiation with Se-PEG-Cur nanoparticles were investigated on melanoma cancer. In this experimental study, nanoparticles of selenium-polyethylene glycol-curcumin (Se-PEG-Cur) were synthesized, and their UV-vis absorption, particle size, zeta potential and photothermal conversion efficiency were determined. Se-PEG-Cur was then introduced as a novel 808-nm laser light absorbing agent as well as ultrasound (US) wave for treatment of C540 (B16/F10) cancer cells. Also, ROS generation in C540 (B16/F10) cancer cells was measured upon PTT and SDT using Se-PEG-Cur. Mean size, zeta potential and photothermal conversion efficiency of Se-PEG-Cur were obtained as ~300 nm, 42.7 mV and 16.7%, respectively. Cell viability upon irradiation of the laser light or US waves with 100 µg mL-1 Se-PEG-Cur were decreased to 33.9 and 22.9%, respectively. Intracellular ROS detection indicated that dual PTT and SDT in the presence of Se-PEG-Cur induced the highest ROS production. Se-PEG-Cur was therefore introduced as an absorbing agent of both laser light and US waves for cancer treatment.