Simultaneously determination of copper and zinc in human serum and urine samples based on amoxicillin drug by dispersive ionic liquid- liquid microextraction coupled to flame atomic absorption spectrometry
In this work the effect of amoxicillin on copper and zinc (Cu and Zn) deficiency was evaluated by determining of Cu and Zn concentration in human serum and urine samples. By dispersive ionic liquid cloud point extraction procedure (DIL-CPE), 0.03 g of pure amoxicillin drug was added to mixture of 0.1 g of hydrophobic ionic liquid and 0.2 mL acetone which was injected to 2 mL of serum or urine samples which was diluted with DW up to 10 mL. The cloudy solution was shacked for 7 min and Cu and Zn ions was extracted based on sulfur group on amoxicillin ligand at pH of 7 by DIL-CPE. Then, the solution centrifuged and after back extraction with I mL of nitric acid (0.2 M), the remained solution was determined by flame atomic absorption spectrometry (F-AAS). The enrichment factor (EF), LOD and linear range (LR) for copper and zinc was obtained (9.92; 9.81), (28.5 μg L−1; 15.2 μg L−1) and (100 -505 μg L−1; 41- 153 μg L−1), respectively. The results showed us, the concentration of the Cu and Zn ions can be decreased by increasing amoxicillin drug dosage in human body. The mean value for serum copper/zinc ratio was obtained 1.11 ± 0.28. The DIL-LME method was validated by ICP-MS analysis and spike of real samples for Zn and Cu ions in serum and urine samples.
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