The effect of different concentrations of sodium di-formate on serum biochemical and antioxidant indices in Salmo trutta caspius

Caspian brown trout (Salmo trutta caspius) is one of the nine subspecies of brown trout in the world and is one of the economically and endangered species of Iran. This study aimed to investigate the effect of sodium diformate as an acidifier on biochemical and antioxidant indices in Caspian salmon. For this purpose, the Caspian brown trout fries (salmo trutta caspius) that weight 13±0.05 gr were recruiting for 60 days by employing four diets consisting of levels of zero (control), 0.5, 1 and 1.5 percent with 3 repeats and blood sampling was done at the beginning of the experiment, day 30 and after the end of the test. Biochemical methods were used to determine the activity of catalase, superoxide dismutase (SOD) and malondialdehyde (MDA) enzymes, as well as triglyceride, cholesterol, LDH lactate dihydrogenase and glucose levels. The results showed that the amount of glucose in the first day of all treatments significantly differed from the control group. The amount of serum cholesterol on 30 days of the 0.5% treatment and 60 days of the 1% treatment was significantly decreased in contrast with the control. The triglyceride concentration in all of the groups except the 1% treatment was higher than the beginning of the experiment. Besides, there were significant differences between 0 and 30 days and on day 60 of 0.5 and 1.5% treatments. LDH enzyme level was significantly decreased on day 60 of the 1% treatment when compared to control group. Serum catalase level was not significantly different from the control group on day 60. SOD level after 60 days was not significant compared to the control group. The amount of malondialdehyde (MDA) activity on days 30 and 60 of the 1 and on day 60 of the 1.5% treatment was significantly different from control. The results of this study showed that the acidifier treatments (1% and 1.5%) had a significant effect on the biochemical factor and antioxidant defense of brown trout caspius for the first 30 days but in the second 30 days the results were the opposite.