Sequencing and characterization of promoter components 26Sp7 gene and expression pattern in wheat under drought

Drought is one of the most important abiotic stresses in agriculture, which limit crops production. Drought tolerance is a complicated trait that is controlled by polygenes, which is expresses the difficulty of breeding of drought tolerance. Variety of strategies has been used to improve this trait in crops, including traditional selection methods and genetic engineering. In this case, irradiation is an appropriate approach for improving the level of genetic variation in a short time. Many studies show that the expression of most genes in environmentally variable conditions is based on the combination control of several transcription factors and their binding to the elements in the promoter. Therefore sequencing of one of the genes involved in the degradation of proteins (26Sp7) using genome sequencing, sequencing of promoter using NEW PLACE and plantCARE software, And the expression pattern 26Sp7 in mutant line T-65-58-8 (semi-drought tolerant) derived from Tabasi (drought-sensitive) radiated with gamma rays in seedling stage during drought stress by QRT-PCR were studid. The expression of the 26Sp7 in Tabasi and mutant showed an increase in all stress treatments. So, in the mutant line, it has peaked in 3 hours after start of stress. On the other hand, in Tabasi, with the onset of drought stress, the expression of this gene increased, until it reached maximum in 48 hours after stress. The expression in the Tabasi showed an increase in gene expression in all stress treatments, which in turn probably indicates the destruction of proteins in drought stress treatments, which also verified the sensitivity of this variety. on the other hand, the promoter sequences of this gene indicate regulatory elements during drought stress such as dehydration responsive elements (DRE) and the binding site of MYB and MYC proteins and abcisic acid responsive elements (ABRE) and responded to light (GT) and gene transcription regulator elements such as the TATA box and the CAAT box. Thus, the increased expression of this gene during drought stress may be attributed to the binding sites of transcription factors that cause this gene expression during drought.