Bioinformatic-based Identification of MicroRNAs with Biomarker Potential in Colon Cancer from Microarray Data
Colon cancer is a common disease in the world that causes high mortality in affected people. The lack of appropriate diagnostic and prognostic markers has led to the failure in early diagnosis of colorectal malignancies. MicroRNAs play an important role in controlling the expression of target genes involved in the development and progression of colon cancer. The aim of the present study was the bioinformatics identification of microRNAs with distinct expression in cancerous and non-cancerous colon samples.
This type of study was theoretical bioinformatics and microarray data of 1513 colon cancer samples with the accession number of GSE115513 were obtained from the GEO site and marker genes were selected by using R program. Target genes of the identified microRNAs were provided by TARGETSCAN software and finally, the graphical network was plotted in Cytoscape software.
Analysis of microarray data showed that has-miR-663b, has-miR-650, has-miR-17-5p, has-miR-4539 and has-miR-501-3p have biomarker potential in cancer samples. Statistical analysis and investigation of the target genes indicated that miR-663b (ROCAUC=0.8965, p=0.001) and has-miR-650 (ROCAUC=0.9104, p=0.001) had significant distinct expression between cancerous and non-tumor margins with biomarker potential.
The has-miR-663b and has-miR-650 genes can be used as diagnostic markers to distinguish colon cancer from non-cancerous samples
-
Identification of oncogenic microRNAs and their target genes in tissue samples of colorectal cancer: Gene set enrichment analysis
, Matin Vafaei *, Mohammad Maroufi, Sheida Emamirad
Micro Nano Bio Aspects, Summer 2024 -
Plasma microRNAs as non-invasive biomarkers in bovine endometritis caused by Gram-negative and Gram-positive bacteria
Arman Shokri, Reza Asadpour *, Razi Jafai-Joozani, , Abolfazl Hajibemani, Gholamreza Hamidian
Veterinary Research Forum, Aug 2023 -
Novel formulations of Kaempferol and its monosaccharide derivatives for healing cancers and microbial infections
Nasser Karimi *,
Micro Nano Bio Aspects, Summer 2023 -
Bioinformatic Prediction of Non-Coding Genes related to the Mouse FGF8, NOG, and BMP4 Ectodermal Differentiation Pathway Genes and Mapping of Related Network
Somayeh Moghaddam, *
Ilam University of Medical Science, -
Tumor Suppression or Oncogenic Role of miR-100 in Cancer; Implications for Esophageal Cancer
Lida Nariman, Saleh, Fam, Milad Bastami, *
Govaresh, -
The Features of Esophageal Cancer in Ardabil Province:Report of a Population-based Cancer Registry in Northwest Iran
Farhad Pourfarzi, Manouchehr Barak, Masoud Babaei, Giti Rahimi, Reza Malekzadeh, *
Govaresh,