Determination of the Young Modulus of Gastric Cancer Tissue Experimentally Using Atomic Force Microscopy

Diagnosis of cell properties to isolate healthy and damaged tissues of biological cells, moving and manipulating cells and various micro/nanoparticles, imaging and diagnosis the shape of cells and different surfaces are new applications of atomic force microscopes that today use atomic force microscopes. In modeling the manipulation of micro/nanoparticles, using atomic force microscopy, one of the important points is to use a suitable and accurate contact model. Since in three-dimensional manipulation, the micro/nanoparticle is located between the beam and the substrate, contact theories must be divided into two parts. The first part is the contact between the base plate and the micro/nanoparticle and the other part is the contact between the micro/nanoparticle and the tip of the beam tip. In this study, the modulus of elasticity of gastric cancer tissue was extracted using atomic force microscopy to diagnose tissue. For this purpose, two Hertz and JKR contact models have been developed to derive the modulus of elasticity. In an experimental experiment, after isolating the cells from the gastric cancer tissue, the specimens were tested using a rectangular beam and pyramidal and spherical tips under an atomic force microscope, and the load-indentation depth diagrams were obtained. Data analysis was performed. According to the results obtained from the load-indentation depth diagram, the apparent spring coefficient for the cell can be modeled because the slope of this diagram is linear. The modulus of elasticity of the desired texture, according to the obtained diagrams obtained from the comparison of the experimental results extracted from the atomic force microscope and the results of the Hertz and JKRcontact theory of 325 ± 255 kPa, has been obtained.