Effect of supplementary irrigation and seed inoculation with bio fertilizers on yield and some physiological traits of rainfed wheat (Rasad cultivar)

In order to study the effects of supplementary irrigation and seed inoculation by bio fertilizer on yield and some physiological traits of rainfed wheat (Rasad cultivar), a factorial experiment was conducted based on randomized complete block design with three replications in Agricultural Research Station of Ardabil in 2016. The factors experimental were included supplementary irrigation at three levels, once supplementary irrigation at booting stage (I1), once supplementary irrigation at heading (I2) and no irrigation as rain fed (I3), and biofertilizers in four levels without application of bio fertilizers as control (B1), seed inoculation with Azospirilum (B2), mycorrhizal application (B3), both application mycorrhizal and Azospirilum (B4). Means comparison showed that application of both bio fertilizers (mycorrhiza and Azosprilium) and once supplementary irrigation at booting stage and once supplementary irrigation at heading increased the grain yield about 35.27 and 28.9% respectively in comparison with application of bio fertilizers under rainfed conditions. Both application mycorrhiza and Azosprilium and once supplementary irrigation at booting stage increased maximum fluorescence (Fm), variable fluorescence (Fv), chlorophyll index and relative water content of flag leaf in comparison with no application of bio fertilizers under rainfed condition. Rainfed conditions increased electrical conductivity and minimum fluorescence (F0).