A comparative study ofthree types oftitanium dioxide nanoparticles effects on including cell growth inhibition in T47-D breast cancer cells and DNA Interaction

Nowadays, titanium dioxide nanoparticles (TiO2NPs) are being consumed in the nanotechnology industry and medicine and are synthesized in various ways. The purpose of this study was to investigate the effect of the surface coating of TiO2NPs on their ability to initiate structural disorder in DNA along with their ability to induce growth inhibition in T47-D cells. The binding constants indicated that TiO2NPs with ethylene glycol dispersion matrix interacted with TiO2NPs with a higher binding constant value (2.2105 M -1 ) compare with two other TiO2NPs (2.3104 M -1 for uncoated TiO2NPs and 1.1105 M -1 for TiO2NPs contains HNO3 stabilizer). CD spectra revealed TiO2NPs have a significant influence on the DNA base stacking. With MTT assay, the IC50 value for each NPs was calculated. DAPI staining and flow cytometry analyses confirmed that TiO2NPs with ethylene glycol dispersion matrix (IC50=63 µM) could reduce cell proliferation strongly, in comparison with two other TiO2NPs. This study indicated that the surface coating of TiO2NPs could affect the value of their interaction with DNA and change their growth inhibition ability. Consequently, the type of coating of nanoparticles is very important in designing nano-drugs, especially for cancer treatment.