Improvement of Incubation Conditions, Buffer System and the Effect of Airflow on Kinetic Growth Rate of Theileria annulata Schizont Infected Bovine Myeloid Cell.

Kinetic growth rate of the Theileria annulata (Tannualata) schizont infected monocyte-macrophage cell (SIMC) using modified Stoker medium in different incubation conditions was studied. In this regard 28 Roux tlasks were divided into four groups each of 7. Two groups (one with HEPES and the other without HEPES) were incubated with carbon dioxide tlow. The reminder two groups were incubated under conventional condition. Ali bottles were observed for viability test and pH changes daily up to 7 days. To study the effect of airtlow on cell growth rate sorne glass tubes with different diameter were inserted through stopper of Roux bottles containing cultured SIMC. The results indicate that carbon dioxide tlow together with HEPES have an increasing effect on cell quantity moreover, the specifie growth rate index of ail groups were similar. The different amounts of airtlow doesn't have any effect on oxygen consumption, therefore the routine culture method using the trapped air in bottles, is the most suitable method for making a suspension culture of Tannulata SIMes.