Immunogenicity and Efficacy of Baculovirus Derived Glycoprotein D of Herpes Simplex Virus Type-I in Mice

The recombinant glycoprotein 0 (gO) of herpes simplex virus type-I (HSV -1) in baculovirus expression system was produced. Spodoptera Frugiperda cell, clone 9 (Sf9) was cultured in modified Grace's medium and inoculated with 5- 7 multiplicity of infection of HSV-I recombinant baculovirus carrying gO gene. Inoculated cells were harvested 96h post inoculation and treated with 3- [(3-cholamidopropyl)dimethylammonio ]-I-propanesulfonate (CHAPS) and phenylmethylsulfonyl tluoride (PMSF) and sonicated at 20KHZ for 5 times. A western blotting was developed and applied to detect the prepared protein. Three groups of BALB/c mice each of 7 mice were inoculated with the recombinant gO, sublethal dose of challenge virus (104STCIDso) and PBS respectively. Ali inoculated mice were challenged with IOMLOso (106sTCIDso) of the wild IISV-1. Ali mice who had received the recombinant gO survived while 14.3% and 71.5% of mice inoculated respectively with either sublethal dose orthe virus or PBS died.