Isolation and Identification of Infectious Bronchitis Virus from Commercial Chickens

Five infectious bronchitis viruses (lRV) were isolated from commercial chickens. One of them was isolated from a layer flock vaccinated with H 120 on day old and the others were originated from broiler flocks without receiving the vaccine. Ali the isolates caused embryo mortality and or dwarfing, stunting, curling. clubbing of the down and urate deposits in the.. kidney. In tracheal organ cultures (TOCs), the isolates caused typical ciliostasis. Electron microscopy confirmed ail the isolates have Coronavirus morphology. The isolates were also confirmed by dot-immunobinding assay using polyclonal antibody to IBV. In one-day-old specific pathogen free chicks, ail the isolates could induce typical respiratory di stress of lB at 36- 48h postinoculation by intraocular route. Virus-neutralization test showed that two isolates were recovered from broiler flocks did not neutralize by monospecific Massachusetts (Mass) antiserum. indicating the presence of other serotypes in commercial chicken flocks in Iran.