Molecular Cloning of VPI gene of Foot-and-Mouth Disease Virus Type 01/Iran

Double stranded cDNA coding for the immunogenic capsid prolein VPI of foot-and-mouth disease virus type Ol/Iran from the virion RNA was prepared .Two primer pairs for VPI gene of virus were designed by comparison ofpublished sequences and statistically analyzed using oligo and DNasis software. A PCR prograrn was developed and optimized to arnplify a segment of about 645 bp. Noninfected cells were used as negative control. The synthesized cDNA was then c10ned into the NdeI and EcoR 1 restriction enzymes site of pEt-23a+ plasmid using already attach linkers. The plasmid has been successfully propagated in and isolated from Echerishia coli bacterial culture and c10ning of VPI gene was confirmed by Dot Blot hybridization.