A comprehensive overview on biocompatibility activity of MFe2O4 (M = Mn, Ca, Zn) as a nanocarrier of breast cancer drug synthesized by heat treatment method

In this research, manganese ferrite (Mn), calcium ferrite (Ca), and zinc ferrite (Zn) nanoparticles were synthesized by thermal treatment method. Curcumin (CUR) loading and release rate of Mn, Ca, and Zn nanocarriers were investigated at different pHs. The drug loading percentage in Mn nanocarriers were higher than Ca and Zn, which is the result of neutral manganese ferrite in the loading medium. Strong π-π interaction between CUR and manganese ferrite nanocarriers increases drug loading percentage. By changing the pH from 7.4 to 5.5, the release of curcumin from Mn, Ca and Zn nanocarriers increased from 41, 24.7, and 44% to 92, 58.7, and 53%, respectively. The biocompatibility of Mn, Ca and Zn nanocarriers were determined using the MTT method, hemolysis test, and lethal dose test. Studies have shown that although a high percentage of MCF-7 cancer cells are destroyed in the presence of Zn nanocarriers, these nanocarriers have a destructive effect on normal HEK-293 cells.