Optimization of the growth conditions of some of the most important microalgae purified from Bazangan Lake and evaluation of their chlorophyll and carotenoids content

Microalgae are known as a potentially sustainable source of feedstock for fuel, food, chemicals, feeding animals and even for the pharmaceutical industries. In this project, the effects of mediums including (F2, Walne, BG11, BBM and TAP), pH (6.0, 6.5, 7.0, 7.5, 8.0 and 9.0) and light intensity (at five levels: 2000, 2700, 3300, 4500 and 7800 Lux) on growth of microalgae purified from Bazangan lake were studied. The Microalgae growth was determined by measuring dry weight and specific growth rate. Also, chlorophyll and carotenoid content were estimated using the standard biochemical methods in optimal codition. The results showed that in Tetraselmis sp., Nodularia sp., Chlamydomonas sp. and Chlorella sp. microalgae, the highest growth rate was observed in F2 (1.76 gL−1), Waln (0.83 gL−1), TAP (1.28 gL−1) and BG11 (1.72 gL−1) medium, respectively. Also, the optimum pH and light intensity for growth of the microalgae was 7.5 and 4500 Lux respectively. The highest chlorophyll and carotenoid content was obtained in Chlorella sp. with 21.92 μg/ml and Tetraselmis sp. with 6.78 μg/ml respectively.