Evaluation of some biochemical characteristics of medicinal plant basil (Ocimum basilicum L.) under the application of growth phytohormones and phytohormones-like

Recently, plant-derived smoke has been included in the group of growth phytohormones due to its bioactive compounds on biological and biochemical processes. In order to evaluate the phytohormones-like effects of Papaver rhoeas L. smoke derived on some biochemical characteristics of medicinal plant basil an experiment was conducted with repeated measurements in research greenhouse of Campus of Agricultural and Natural Resources of Razi University in 2017. The Experimental treatments included eight levels of leaf foliar application (control, Smoke-water in 100, 5000, 1000 and 5000 dilutions accompanying with cytokinin, auxin and gibberellic acid (each of them with concentration 50 µM)) with 3 replications and 2 harvest stages. The results indicated that application of smoke-water (1:500 and 1:100 (v/v)) improved gas exchange status and also increased chlorophyll content, without significant difference with cytokinin. All chlorophyll fluorescence parameters also increased significantly under the application of phytohormones and smoke-water. Levels of foliar (especially SW: 1:500 and 1:100 (v/v)) reduced electrolyte leakage of plasma membrane and content of soluble carbohydrates. The highest total phenolic and anthocyanins content was obtained from leaf foliar in the smoke-water of 1:100 and 1:500 (v/v), respectively. Smoke-water at concentration 1:100 (v/v) without significant difference with phytohormones auxin and gibberellic acid significantly increased the activity of peroxidase. In general, it can be concluded that aqueous extracts of plants-smoke derived (especially SW 1:100 (v/v)) without significant differences with synthetic phytohormones have the ability to reduce the biochemical limitations of basil and can improve its growth conditions.