Green Synthesis of Silver Nanoparticles Via Ganoderma lucidum Fungus Extract and its Antibacterial Effects on Klebsiella Pneumonia Isolates from Urinary Tract Infections

Urinary tract infection is one of the most important infectious diseases and can be seen at all ages; it is also a major medical problem. Due to the increased antibiotic resistance and high rate of recurrence of the disease, it can cause serious damage to the health of patients. Ganoderma leucidum is one of the most effective fungi with numerous therapeutic properties and is the most effective and best fungi with healing properties. Metal nanoparticles are important in various fields, including medicine, industry, etc. Silver nanoparticles are of particular interest due to their good conductivity, chemical stability, and many other properties.

In this study,30 specimens of positive cultures with urinary tract infection referred to Imam Reza Hospital Laboratory in Bojnourd were studied. Resistance and susceptibility of the isolates were determined by disk diffusion method. In this study, the antibacterial effects of silver nanoparticles were investigated using aqueous extract of Ganoderma leucidum by microdilution method. Vegetative electron microscopy was used to measure the size and shape of silver nanoparticles. FTIR analysis was also performed to investigate possible organic compounds that could interfere with the synthesis of nanoparticles.

The highest antibiotic resistance was related to ampicillin (93.33% /28 isolates). The resulting nanoparticles were 20 to 45 nm in size.

The produced nanoparticles have antimicrobial activity and can be a good alternative in the treatment of antibiotic resistant infectious diseases.