Assessment of the Relative Level of TNF-α Gene Expression in RAW264.7 Cells

Recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. Elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. On the other hand, the role of TNF-α is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. Therefore, in the current study the effect of elaidic acid on TNF-α gene expression, was investigated.  

RAW264.7 cells were treated with 100 and 200 μM of elaidic acid for 12 h. Then, expression of TNF-α gene was assessed after RNA extraction and cDNA synthesis. 

 TNF-α gene expression in RAW264.7 macrophage cell line showed no significant difference for both concentrations compared to the control. 

These results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through TNF-α nuclear receptor.