The Expression Level of CCDC26 and FOXCUT Genes in AcuteLymphoblastic Leukemia

Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells affecting both children and adults. Long non-coding RNAs (lncRNAs) are one kind of non-coding RNAs (ncRNAs), reported modulatingthe initiation or progression of diversecancers. However, the role of CCDC26 and FOXCUT long non-coding RNAs in ALL has been unknown.In this study, we explored the expression of FOXCUT and CCDC26 lncRNAs in acute lymphoblastic leukemia cell lines.

Acute T lymphoblastic leukemia cell lines, RPMI 8402, Jurkat, B lymphoblastic leukemia, Daudi,and Ramoscell lines were used.After culturing the cells, RNA extraction and cDNA synthesiswere performed.The real-time PCR technique was then used to study the expression of CCDC26, FOXCUT, C-kit, and FOXC1 genes.

We found a significant increase of CCDC26 expression in RPMI 8402 (p<0.0001) and Ramos (p<0.05)cell lines compared to the control, while decreased expression of thesegeneswas observed in Jurkat and Daudi cell lines. Furthermore, FOXCUT gene had a significant increase in expression in all cell lines compared to the control (p<0.01 in Daudi and RPMI 8402 cell lines) (p<0.001 in Jurkat and Ramos cell lines).

Our results demonstrated that CCDC26 and FOXCUT genes can play a regulatory role in acute lymphoblastic leukemia and may serve as a potential diagnostic biomarker and therapeutic target of acute lymphoblastic leukemia.