α-glucosidase inhibition activity and antioxidant properties of aerial parts methanol extract from Silene Ampullata Bioss and Campanula Involucrate Auch.ex Dc.

An after meal increase in blood glucose for diabetics is a serious problem. One of the ways to cope with this problem is to inhibit the intestinal α-glucosidase. The inhibitory effect of methanol extract from aerial parts of Silene Ampullata Bioss and Campanula Involucrate Auch.ex Dc. previously has been reported, the purpose of this study was to determine the location of the organs of this activity. The maximum inhibitory activity of Silene Ampullata Bioss, was related to the 2 mg/ml concentration of the leaf organ and flower organ's methanol extract (100%), and again the 40 mg/ml concentration of the leaf organ and flower organ methanol extract produced the maximum inhibitory activity for Campanula involucrate Auch.ex Dc. (100%). The minimum amount of IC50 (0.13 mg/ml) was achieved for the flower and leaf of Silene Ampullata Bioss. According to the results of kinetic studies, the methanol extracts from flower and leaf organs of Campanula involucrate showed non-competitive and mixed pattern of inhibitory activity on alpha-glucosidase, respectively. While the extracts from flower and leaf of Silene Ampullata Bioss showed a mixed and uncompetitive inhibition pattern of inhibitory activity on alpha-glucosidase, respectively. The methanol extract of Silene Ampullata and Campanula Involucrate flower organs had a significant antioxidant activity in comparison with other aerial organs.The methanol extract of the leaf and flower organs of Campanula involucrate and Silene Ampullata possesses effective inhibitors, therefore isolation and purification of active components and in vivo study of their effects on alpha-glucosidase could be the subject of further studies.