Effect of different population of some probiotic bacteria in amount of defense enzymes, microelements uptake, chlorophyll and carotenoids in pistachio

Colonization and population of antagonistic bacteria on rhizosphere have a definite role on their antagonistic effect. In this study in order to determine optimal population density of antagonistic bacteria, in a way that they would be able to induce maximal defensive power in plant, and also in order to investigate effect of initial inoculated population and amount of plant defense enzymes, microelements uptake, chlorophyll and carotenoids; level of peroxidase, polyphenol oxidase, phenylalanine ammonia lyase, total phenolic compounds, total protein, Zn, Fe, Mn, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids in population of 104, 106 and 108 of four antagonistic bacteria Pseudomonas fluorescens VUPF5, P. fluorescens T-17, Bacillus subtilis V1 and B. subtilis 96 in Sarakhs cultivar of pistachio was measured. Experiments were done in randomized complete block design with three replications per each treatment. Studies showed in the measured factors despite of diverse results in bacterial populational treatments, all populational treatments follow a constant pattern in a way that along with increasing in bacterial population, amount of measured enzymes, total phenol content, microelements, chlorophyll and carotenoids increases.