The effect of six weeks of high intense intermittent exercise on the P53 and SRF in tumor tissue of fe-male mice with breast cancer

Breast cancer is the major cause of mortality for women and is wide spread among them. Angiogenesis plays an important role in tumor progression and metastasis. The effect of exercise training on the inhibition or reduction of angiogenesis in tumor tissue is not clear. Therefore, the aim of present study was to investigate the adjuvant therapy effects of High Intensive Intermittent Training on the expression of angiogenesis factors P53 and SRF in breast cancer tumor tissue.

Sixteen Bulb/c mouses were obtained and randomly divided into two groups of control and High Intensive Intermittent Training- Tumor .After daptation to the environment, murine Adenocarcinoma-type cancer cells were injected into all micses. Two weeks after injection, HIIT group mouses were exposed to treadmill for one week.Then HIIT-Tumor group performed their training protocol in six weeks long, three sessions per week and each session lasting for 60 minutes. Rats were anesthetized and sacrificed 48 hours after completion of the protocol. Tumor tissues were isolated and stored at -70˚C. The RNA extraction steps were performed according to the TRIzol protocol made by Invitrogen Company and cDNA was made according to cDNA synthesis kit instruction. Real Time - PCR steps were performed according to Saber-Green instructions and data were collected.

The study data showed that, HIIT decreased SRF by 5.27-fold and caused a 5.97_fold increase in P53, compared to the control group (P ≤ 0.01).

HIIT exercise suppresses tumor growth and inhibits cancer progression by reducing SRF and increasing P53.