Study of Cytotoxicity and Genotoxicity of “Nanoamorphus Calcium Phosphate (NACP)” In Macrophage RAW264 cell line: An In-vitro Study

The aim of study was the biological assessment of cultured RAW264 macrophage exposed to nano amorphous calcium phosphate particles by analyzing of cytotoxicity and genotoxicity tests.

Nanoamorphus Calcium Phosphate particles were produced by sol -gel method, then particle size and hemogenicity was analyzed by XRD (X ray Diffraction). Cytotoxicity of nanoparticles was determinated with mouse RAW264 macrophage. The cells cultured in 37°c in DMEM medium 96 part s plates with concentration of 10000 cells in each part for 72 hours, then the medium removed and the second medium added to cells containing different concentrations of Nano particle (0, 200 and 400μg/ml). After 24 hours of incubation, MTT assay and Annexin V were used for assessing cell viability and apoptosis.

The apoptosis insignificantly increased in macrophages with 200 and 400 μg/ml NACP and for cytotoxicity, cell viability for control, 200μg and 400μg groups were 100,107,103 percent.

NACP has no cytotoxicity and genotoxicity, so it can be used as non -toxic and beneficial material for clinical use.