Micropropagation by IBA and Kin and tetraploid induction by colchicine in Phalaenopsis amabilis

One of the most important orchids in the world is Phalaenopsis amabilis which has allocated around 70% of orchid global market. This plant was used as pot plant and cut flower. Protocorm-like bodies (PLBs) explant, MS culture medium, and plant growth regulators; indole-3- butyric acid (IBA) in concentrations of 0, 0.1, 0.2, 0.5 and 1 mg/l and kinetin (Kin) in concentrations of 0, 0.5, 1, 2 and 3 mg/l were used for this orchid. Results showed that the highest leaf length (4.10 cm) in treatment of 1 mg/l IBA along with 2 mg/l Kin, maximum leaf number (5.25) in treatment of 0.2 mg/l IBA along with 1.5 mg/l Kin, and the highest root length (6.85 cm) in treatment of 0.2 mg/l IBA along with 1 mg/l Kin were obtained. PLBs as explant and colchicine in 4 levels (0, 0.05, 0.1 and 0.15%) for 72 h as antimitotic agent were used to induce polyploidy. Result of flow cytometery analysis and morphological and anatomical traits showed that the use of 0.15% colchicine caused to induce tetraploid plants.