Evaluation of PRR11 gene expression changes and its relationship with tumor size in patients with gastric adenocarcinoma

Gastric cancer is one of the most common gastrointestinal tract neoplasms. Because of its invasion, and nonspecific symptoms and signs, the disease is often diagnosed at an advanced stage with short survival. PRR11 participates in the initiation and progression of lung cancer and breast cancer by regulating important genes involved in cell cycles and tumorigenesis. In this research, we studied the expression of this gene in samples of gastric adenocarcinoma.

In this case-control study, 100 samples including 50 samples of gastric tumor tissue and 50 samples of normal tissue from the patients were prepared. Next, RNA was extracted from tissue and cDNA was made. Finally, using quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR), gene expression measurements were performed for PRR11 and GAPDH. Paired t test was used for data analysis. We also performed a data mining study to determine PRR11 expression in gastric cancer patients.

The rate of PRR11 gene expression in tumor samples were significantly increased as compared to normal tumor margins (p<0.001) and 11-fold change up-regulation has been calculated for this gene. Furthermore, the expression level of PRR11 has a positive correlation with gastric tumor size (p<0.001).

Overall, the results of this study showed that the PRR11 acts as an oncogene in gastric cancer and PRR11 targeting could be used as a strategy to counteract the progression of gastric cancer.