LINC01204 Negatively Regulates the Effect of MiR-214 on Lung Cancer Cell Apoptosis, Migration, Invasion and Radio-sensitivity

To investigate whether LncRNA LINC01204 affects lung cancer cell apoptosis, migration, invasion and radiosensitivity by regulating miR-214.

Normal lung cells HBE and lung cancer cells A549 were cultured in-vitro, and pcDNA3.1, pcDNA3.1-LINC01204, pcDNA3.1-LINC01204 and miR-NC, pcDNA3.1-LINC01204 and miR-214 mimics were transfected into A549 cells, respectively. qRT-PCR, flow cytometry, scratch test, and Transwell chamber test, clone formation test, dual luciferase, and Western blot methods were used in this study.

Compared with HBE cells, A549 cells had significantly reduced LINC01204 expression level (P<0.05), and significantly increased miR-214 expression level (P<0.05); transfection of pcDNA3.1-LINC01204 could significantly increase apoptosis rate and Cleaved-caspase3, E-cadherin protein levels (P<0.05), reduce migration healing rate, N-cadherin protein levels and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 1.950, reduce the number of invasive cells (P<0.05). Dual luciferase report experiments confirmed that LINC01204 can bind to miR-214 in a targeted way; co-transfection of pcDNA3.1-LINC01204 and miR-214 mimics can significantly reduce the apoptosis rate and Cleaved-caspase3 and E-cadherin protein levels (P<0.05), improve the migration healing rate, N-cadherin protein level and cell survival fraction (P<0.05) with sensitization enhancement ratio SER at 0.728, increase the number of invasive cells (P<0.05).

LINC01204 overexpression can negatively regulate miR-214 expression to promote lung cancer cell apoptosis and inhibit migration and invasion, thereby enhancing radiosensitivity.