The Effect of Exercise and Folate Nano-Liposomes on D1 and D2 Receptor Gene Expression in the Brain of Alzheimer's Rats

Alzheimer's is a neurodegenerative disorder characterized by memory loss and cognitive dysfunction. Research has shown that blood metal levels and physical activity may be key to injury and possibly Alzheimer's treatment. The aim of this study was to investigate the effects of high intensity interval training (HIIT) and Folate Nano-liposome on the expression of D1 and D2 receptors in the hippocampal tissue of Alzheimer's rats.

Thirty-three male Wistar rats at the age of eight weeks were prepared from Pasteur Institute. Rats were randomly divided into 5 groups (healthy control, Alzheimer's control, Alzheimer+ HIIT, Alzheimer +Folate Nano liposomes, Alzheimer+ HIIT +Folate Nano liposomes).Alzheimer's induction was performed and Folate Nano-liposomes were injected as a supplement .After the last training session, rats were anesthetized and the hippocampus was analyzed.

The results of one-way ANOVA showed a significant increase between the groups in D1 mRNA and D2 mRNA (P≤0.000). The results of the Bonferroni post hoc test showed that there was a significant increase the control group and the Alzheimer's, Alzheimer's + HIIT and Alzheimer's +folate Nano liposomes. There was a significant increase between Alzheimer's group and Alzheimer's+ HIIT and Alzheimer's+ HIIT+ folate Nano-liposomes (P≤0.05).

Resistance training and Folate Nano-liposomes have changed the content of D1 and D2 in the brain after Alzheimer's induction. These changes may be due in part to the synergistic effect of physical activity and Nano-pharmaceuticals in preventing or reducing the detrimental effects of pathological conditions.