Simultaneous spectrofluorometric analysis of caffeic and ellagic acids through inclusion complex formation with γ-Cyclodextrin

Fluorescence spectroscopy due to its high sensitivity continues to be of extensive attention to analytical chemists. Spectra recording at several interval times while the reaction is preceded, or at various concentrations of complexing agent, are cases of policies by which one can create second-order data by the spectrofluorometric technique. In this case, fluorescence spectra of a mixture of two caffeic acid (CA) and ellagic acid (EA) target analytes were recorded as a function of γ-cyclodextrin (γ-CD) (as an inclusion complexing agent) concentration to produce second-order data. At this point, bilinear least squares/residual bilinearization (BLLS/RBL), as a second-order calibration technique, due to its benefits of rapidity, accuracy, simplicity, suitable spectral resolution, and concentration estimation even in the presence of the unknown interference (Second-order advantage), was exploited for deconvolution of trilinear data to obtain fluorescence spectra and concentration profiles of the CA and EA as a function of γ-CD concentrations. A calibration set comprising 10 reference samples was employed to build BLLS/RBL model. The resulting model predictive ability was validated by a test set including 6 samples. The suggested model was effectively exploited to simultaneously quantify the content of CA and EA in 4 fruit juice samples.