The extraction and identification of compounds in sea urchin (Echinodermata mathaei) with Supercritical Fluid Extraction

The existing compounds in a species of sea urchin (Echinodermata mathaei) were identified. The study area was in sub-tidal waters of Bandar- e - lengeh within the geographic east longitude 54° 53.312 and north latitude 26° 32.739. Random sampling took the full tide by diver. In order to identify the components of this organism, five sea urchins with an average length of about 8.5±1 cm and a width of 7±1 cm and a weight of 66.680±10 grams were evaluated. After preparation, samples were extracted by supercritical fluid (Model Suprex MPS/225 Manufacturing Co. America Pittsburgh) and identified by gas chromatography (Model 5890 Manufacturing Co. Hewlett Packard) coupled with mass spectroscopy (Model 5970 Manufacturing Co. Hewlett Packard). Compounds identified in this sea urchin with SFE method under optimal conditions were: 2.85% n-Hexadecanoic acid, methyl ester, 1.9% Octadecanoic acid, 3.03% Cyclo propanenonanoic acid, 77.4% Cholesterol, 6.45% Ergosta-5, 22-dien-3-ol, 5.15% Fucosterol. Among these compounds, fucosterol and n-Hexadecanoic acid had antioxidant and anti-inflammatory properties respectively. Also Ergosta-5; 22-dien-3-ol had the germination properties.