Root Protein Interactomics of Salt Stress-Induced Proteins of Wheat Genotypes KH-65 (Salt-Tolerant) and PBW-373 (Salt-Susceptible)
Author(s):
Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction
Wheat crop is moderately tolerant to salt stress and is considered as an excellent system to study salt stress tolerance despite its genetic complexity. In the present study, the top ten biological processes in a root proteome were mapped for Protein-Protein Interaction (PPI) networks and analyzed to examine the effect of salt stress on wheat cultivars KH-65 and PBW-373.Materials and Methods
NaCl salinity treatment 0 and 300 mM NaCl was performed on a three-leaf stage plant. Roots proteins were analyzed by liquid chromatography-mass spectroscopy. Proteins were grouped according to GENE ontology terms for biological processes and arranged in descending order. Interactome analysis was done through the STRING database.Results
Interacting root proteins of tolerant line KH-65 show a comparatively higher number of nodes, edges, and interacting proteins than the sensitive line, PBW-373. The number of proteins whose expression was positively induced upon salinity stress was significantly higher in the roots of salinity-tolerant KH-65 than that of the PBW-373 roots. Moreover, the fold induction too was also high in the tolerant line. Similarly, the number of participant proteins in an interaction network of the KH-65 roots was higher than that of the PBW-373 cultivar.Conclusions
This analysis provides valuable information in elucidating the molecular mechanism associated with salt stress response in wheat seedlings’ roots. The observation is correlated with the efficient salt tolerance capacity of KH-65. The higher expressing proteins in interaction networks may be seen under the increased salt tolerance capabilities of salt-tolerant KH-65 line.Keywords:
Language:
English
Published:
Journal of Applied Biotechnology Reports, Volume:9 Issue: 2, Spring 2022
Pages:
632 to 639
magiran.com/p2449122
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!