Effects of Gonadotropin Releasing Hormone (GnRHa) and Carp Pituitary Extract (CPE) on Spawning Success, Fecundity, Fertilization Success and Survival Rate in Goldfish Carassius auratus
In this investigation effectiveness of a Gonadotropin hormone (GnRHa) and Carp pituitary extract (CPE) on spawning success, fecundity, fertilization success and survival rate in Goldfish Carassius auratus were studied. The brood stocks of Goldfish were divided to 5 treatments were injected as follows: 10µg/kg BW GnRHa and 20µg/kg BW GnRHa and 3mg/kg BW CPE and 5mg/kg BW CPE. The females were injected in 2 times (with 12 hours distance) and the males were injected once (simultaneous with second injection of females). Finally, in the fishes that received hormones, all of them were ready for spawning, but non of control group’s fishes was ready for spawning. Total achievable eggs in hormonal treatment fishes on sequence in 10 µg/kgBW GnRHa, 20 µg/kgBW GnRHa, 3 mg/kgBW CPE & 5 mg/kgBW CPE were ±211057.81, ±255741.51, ±196255.07 & 65±2429.01. Fertilizibility of the treatments on sequence were1399.2±47.55, 1772.7848±.47, 1179.5±49.19 & 1590.3±38.52. Hatchability of the treatments on sequence were 1206±32.96, 1491.4±36.83, 982.8±53.53 &1395.5±32.23 and the survival rates of larvae were 1092.9±33.41, 1336.17±41.3, 880.62±54.43 & 1230.74±32.89. Results showed that hormonal (CPE and GnRHa) induced propagation of Goldfish was successful. Relative fecundity, fertilizibility, hatchability and survival rates of larvae were not significantly different between treatments 1 and 3 and between treatments 2 and 4 (P<0.05) respectively. Dunnet test was showed significant differences among control and treatment groups.
Propagation , Goldfish , Carassius auratus , GnRHa , CPE
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