Study the Freezing Resistance of some Barley Varieties Using Rates of Electrolyte Leakage

In order to study the freezing resistance of some barley varieties using rates of electrolyte leakage this experiment was carried out on three barley variety, including Reyhan, kavir, and Nosrat. These varieties are the most important varieties that cultivated in Semnan province. For this experiment, barely seeds were  sown in small containers. The current experiment was carried out as Factorial experimental design plan based on completely randomized design (CRD).The first factor in this study was barley variety in three levels. The second factor was temperature in 5 levels including +4°C, -4°C,  -8°C, -12°C and -15°C. Seedlings in  the stage of  tillering, was sprayed  by  water and then  were put in the incubator for two hours. Then two gram  samples  was selected  and 20 mm deionized water was added and shakes for 24 hours. Then every day for a week  EC  in solution was measured. Analysis of variance (ANOVA) was used to determine significant (p < 0.01 and p < 0.05) differences between  barley  genotypes. The  differences  between means of barley varieties  were  inspected using Duncan test . The results showed that the percentage of ion leakage between the three cultivars had   significant differences .The effect of temperature on the percentage of ion leakage  in all days, had  significant difference.The highest percentage of ion leakage measurements on all days was at the  -15°C and the lowest percentage of leakage occurred at 4 °c . Kavir variety  in comparison with other varieties had  lower cold tolerance. Interaction of  temperature and genotype factors on  ion  leakage measurements showed that all varieties had significant differences.