Anti-cancer effects of hydroalcoholic extract of Digitalis nervosa in breast cancer (MCF7) cell line

The Digitalis nevrosa has anti-cancer properties due to its glycosidic compounds The aim of this study was to investigate the anticancer effects of hydroalcoholic extract of digitalis nevrosa on MCF-7 breast cancer cell line. In this experimental study, hydroalcoholic extract of thistle was produced. MCF-7 cells were then treated with different concentrations (3.125, 6.25, 12.5, 25, 50 and 100 μg / ml). The effects of cytotoxicity of the extract were analyzed by MTT method and after determining the concentration of 50% lethality of the extract, the expression changes of P53, Bax, Bcl2 and CDH1 genes were analyzed by real-time PCR. The data were evaluated using SPSS software and one-way ANOVA and Tukey test. The results of MTT showed that the effect of the extract on viability was dosedependent and the highest cytotoxicity was related to the concentration of the extract at 39/45μg / ml. Which were statistically significant were shown to be significant.(P<0.001). Also, the effect of silver nanoparticles with a concentration of 50% lethality of 39/45 μg / ml on the expression of P53, Bax, Bcl2 and CDH1 genes in comparison with the control gene showed significant changes. Digitalis nevrosa extract induces apoptosis in MCF-7 cell line and the use of this plant can be considered as a promising choice in the treatment of breast cancer.