Effect of plant growth regulators on callogenesis and organogenesis in walnut (Juglans regia L.) cultivar Chandler

Walnut (Juglans regia L.) of the walnut family (Juglandaceae) is one of the most important edible species among dry fruits. In order to determine the best callus and organogenesis treatment on walnut leaf and bud explants in the DKW culture medium, a factorial experiment was conducted in the form of a completely randomized design. In the first experiment, leaf explants were cultured in DKW medium, the first factor was 2,4-D (0, 1, 2, 3 mg/l ), and the second factor was TDZ (0, 1, 2, 4 mg/l). In the second experiment, which was performed using bud explants, BAP growth regulator with concentrations of 0, 1, 2, 3 mg/l and IBA with 0, 0.01, 1, 2.5 mg / l were used. In the first experiment, the results showed that the highest number of callogenesis occurs in DKW medium containing 3 mg/l 2.4-D and the highest number of leaf browning occurs in medium containing 2 mg/l 2,4-D and 4 mg/l TDZ. In the second experiment, the highest rate of organogenesis was observed in a culture medium containing 1 mg/l IBA without BAP. The results of this study showed that there is a possibility of callus formation from leaf explants in DKW medium containing 3 mg 2,4-D and organogenesis of bud explants in DKW medium containing 1 mg/l. Also in the treatment containing 2 mg/l BAP and 1 mg/l IBA callus formation of walnut germ explants was observed.