The Optimization of Faecalibacterium prausnitzii Extracellular Vesicle Concentrations on PPARγ and FIAF Expression in the Caco-2 Cell Culture Model

Faecalibacterium prausnitzii is an important member of human gut microbiota with a critical function in the health of humans through the induction of inflammatory and immune responses leading to intestinal hemostasis. Microbiota-induced extracellular vesicles (EVs) were presented as a novel communication pathway between microbiota and the host that are capable of imposing positive effects on the host. Recently, EV-based treatments have been evaluated in different studies, and using EVs derived from microbiota has been recommended in recent studies as post-vaccination adjuvants. Accordingly, in the present study, the effects of various EV concentrations on the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ) and fasting-induced adipose factor (FIAF) genes were evaluated and optimized in the human epithelial colorectal adenocarcinoma (Caco-2) cell line.

The effects of the extracted EVs from F. prausnitzii on the PPARγ and FIAF gene expression were investigated in the Caco-2 cell line via cell culture and quantitative real-time PCR. The obtained outcomes were then compared with those of our previous study (concentrations of 50 and 100 µg/ml).

It was shown that F. prausnitzii EVs (150 µg/ml) significantly increased PPARγ and FIAF gene expression in the Caco-2 cell line, relative to previous studies conducted by our team.

Considering the positive impact of F. Prausnitzii EVs on the studied genes’ expression in the present study, the EVs of the bacterium will be proposed as new post-vaccination adjuvants in people suffering from intestinal barrier disorders such as inflammatory bowel disease patients. However, more studies should be performed in this respect.