Load Modeling of The Pulsed Power Generators for Electroporation Using Impedance Spectroscopy of Human Lung Normal and Cancer Cells

In this paper, the load of the pulsed power generators is modeled using the impedance spectroscopy of human lung normal and cancer cells for electroporation. Due to the differences in the electrical behavior of normal and cancer cells, cell modeling can be used in cancer diagnosis and treatment selectivity (causing cell death in cancer tissues and not damaging the cells, and normal tissues) is effective. Also, due to the wide changes in cell impedance under different conditions and the dependence of pulse generator structures on load impedance, cell modeling can have a significant effect on the design of pulse generators and the creation of pulse parameters with the greatest effect. In this paper, the impedance of biological samples is measured at different frequencies. Finally, for quantitative analysis of the electrical properties of cells, an accurate electrical equivalent circuit is provided for the load. The proposed electrical model is not cell-focused but on the set of cuvette and its internal contents. This electrical model, taking into account the effect of the two-layer electrical capacitor and parasitic effects, and expanding the frequency range from 100 Hz to 500 MHz. The results show that the equivalent electrical circuit provided in most cases with an low error is consistent with the impedance measurements performed for different samples.