Survey of Two Methods Including Phenol-Chloroform and Application of Magnetic Nanoparticles for Genomic DNA Extraction of Staphylococcus aureus

Isolation of genomic DNA from bacterial cells is one of the processes typically performed in most biological laboratories and there are different methods to do it .In this study, two methods including phenol-chloroform, and magnetic nanoparticles, were used to extract genomic DNA of Staphylococcus aureus.

In the present study, the standard strain of Staphylococcus aureus ATCC 25923 was used for extraction of genomic DNA by phenol-chloroform and magnetic nanoparticles methods. Nanodrop and electrophoresis on agarose gel were used to evaluate the quality and concentration of extracted DNA.

The concentration of extracted DNA by phenol-chloroform and magnetic nanoparticle) SiO2/Fe3O4) methods were obtained 550.4 and 131.6 µg/ml respectively.

From the findings of this study it can be concluded that due to the thick wall of Staphylococcus aureus, genomic DNA extraction by magnetic nanoparticles (Fe3O4/SiO2) have acceptable concentration and purity for molecular processes such as PCR, and can be used as an alternative to other extraction methods.