Effect of Vincristine on miRNA-15a expression in Epstein‐Barr‐Virus transformed B cell-CO 88BV59-1 LCL

Despite advances in diagnosis and therapy, cancer is still the main cause of death in world. Vincristine is one the major drug in tumor treatment. However, the anti-lymphoma effects of Vincristine on micro-RNA expression are not clear, especially in B cell lymphomas. The aim of the present study was to evaluate the effects of Vincristine on miRNA-15a3p and miRNA-15a5p genes expression Epstein‐Barr‐Virus (EBV)-infected transformed B cell lymphoma. In this study, the effects of cyclophosphamide on CO 88BV59-1 LCL – an EBV infected transformed B cell was evaluated. The cells were treated with Vincristine (0.05-50 µM), for 24-72 hours. MTT, flow cytometry and real-time PCR techniques were used for cell viability, and cytotoxicity evaluations and miRNA-15a genes expression, respectively. Vincristine significantly inhibited proliferation and induced cell death in EBV infected cell-line in a dose and time-dependent manner (P<0.05). There were no significant changes in the expression of miRNA-15a3p and miRNA-15a5p in treated cells compared to untreated cells (P>0.05). The results showed that cytotoxic effects Vincristine on CO 88BV59-1 LCL is independent on miRNA-15a3p and miRNA-15a5p expressions.