Optimal methods for the preparation of fungal mycelium for examination with the scanning electron microscope

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Article Type:
Research/Original Article (دارای رتبه معتبر)
Abstract:
Introduction

Plant-pathogenic fungi are considered a serious threat to world food security, causing spoilage in plant products and food poisoning. One of the ways to identify fungi is to examine their mycelium using a scanning electron microscope (SEM). In order to take high-resolution microscopic images and obtain the surface properties of mushroom mycelium samples, it is very important to prepare the samples, i.e. the method of dehydrating and drying them.

Materials and Methods

In this research, the effect of two dehydration methods and two drying methods on the mycelia of two fungi, Rhizoctonia solani and Rhizopus stolonifer, were compared to study them with SEM.

Results

Microscopic observations showed that for R. stolonifer, dehydration with 100% ethanol and drying and for R. solani at room temperature and dehydration with 50% ethanol and the freezing method produce a smooth and high-resolution image compared to other methods.

Conclusion

Dehydration of fungal mycelium with 50% to 100% ethanol and drying at room temperature or freezing are the optimal methods to prepare them for examination with SEM and produce a smooth and high-resolution image, and the surface features of their mycelium are preserved and well-defined.

Language:
English
Published:
Plant Pathology Science, Volume:11 Issue: 2, 2022
Pages:
42 to 51
https://www.magiran.com/p2526084  
سامانه نویسندگان
  • Vakili Ghartavol، Masoumeh
    Author (1)
    Vakili Ghartavol, Masoumeh
    .Ph.D Agriculture, Ferdowsi University, Mashhad, Iran
  • Golmohammadzadeh، Shiva
    Author (3)
    Golmohammadzadeh, Shiva
    Professor Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
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